BD Fortessa X20 is a state - of - the - art analyser equipped with 5 spatially spectrum is covered by optical filters, however if you need different filter setup for

LSR Fortessa . The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time! Technical Highlights Lasers 355 nm, 20 mW laser power 405 nm solid state diode, 50 mW laser power 488 nm solid state, 50 mW laser power 561 nm, 640 nm , 40 mW laser power Flow cell 180 x 430µm rectangular quartz flow cell

(ALDH hi ) detekterades av BD Fortessa cellanalysator (BD Biosciences). im filter (BD Biosciences), blandade med de uppsamlade supernatanterna. on FACS LSR Fortessa and LSR II flow cytometer (BD Biosciences) and data Fortessa flödesanalysator (BD Biosciences) användes för TagBFP mättes med användning av en 405 nm laser och ett 450/50 filter med ett fotomultiplikatorrör Spinnkoppar köptes från ThermoScientific och tillfördes 10 μm filter bifogade medan Fluorescens analyserades med användning av BD LSR Fortessa Flow Prover kördes i en LSR-II X-20 Fortessa (BD Biosciences, San Jose, CA) vid South PA) och med användning av TRITC (för Alexa Fluor 594) och DAPI-filter. Cellpelleten blandades med Matrigel (BD Biosciences) och 10 000 celler 100 nm pore size polycarbonate filters (Northern Lipids) using nitrogen pressure and a avseende på GFP-uttryck genom flödescytometri (Fortessa, BD biosciences). The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

Bd fortessa filters

Sample loading via tubes. Table of lasers and filters available: Laser Filters 488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter. In Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company.

BD FACSCelesta ™ multicolor cell analyzer . The BD FACSCelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package. With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research.

4. Remove the sheath container lid.

2013-06-27 · With the industry's most advanced high throughput plate loader and hotel, the S1000 family of analyzers go head-to-head with BD FACS Canto, BD LSR II, and BD Fortessa at a fraction of the cost and size while offering a superior solution for conventional and high throughput flow cytometry applications.

Clone. Cat. No. Blue. 488 nm. 530/30. FITC.

The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm). BD™ Brilliant Violet™ 510 Bandpass Filter (525/50BP) Size:1 Each Status:RUO Cat No.:656739. Quick View.
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BD LSRFortessa™ Cell Analyzer; Special Order BD LSRFortessa™ Cell Analyzer Product Information Sheets. BD™ Optical Filters: Optimal detection of BD Horizon™ Brilliant Violet™ Polymer Conjugates Quick Reference Guides. BD FACSDiva™ Software v8.0 Administrative Tasks 2018-12-11 · Default Filters for Configuration BD_LSR-Fortessa_18-COLOR_20181211 Laser Detector Name Dichroic Mirror Bandpass Filter Sample Parameters / Fluorochromes BD LSRFortessa™ Cell Analyzer Filter Guides. BD LSRFortessa™ Cell Analyzer; Special Order BD LSRFortessa™ Cell Analyzer Product Information Sheets. BD™ Optical Filters: Optimal detection of BD Horizon™ Brilliant Violet™ Polymer Conjugates Quick Reference Guides.

BD™ Optical Filters: Optimal detection of BD Horizon™ Brilliant Violet™ Polymer Conjugates Quick Reference Guides. BD FACSDiva™ Software v8.0 Administrative Tasks BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 ** Additional filter changes can be requested. BD- LSR Fortessa Configuration Violet (405nm) Blue (488nm) Yellow-Green (561nm) Red (640nm) Author: Julien Leconte BD Horizon™ BUV737 740/35 690LP 659997 BD Horizon™ BUV805 820/60 770LP 659998 Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon™ BV421 450/40 656738 – – BD Horizon™ BV510 525/50 656739 505LP 656745 BD Horizon™ BV605 610/20 656740 595LP 656746 BD Horizon™ BV650 660/20 656741 630LP 656747 BD Horizon™ BV711 710/50 656742 Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).
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Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3.

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The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) Default Filters for Configuration BD_LSR-Fortessa_18-COLOR_20181211 Laser Detector Name Dichroic Mirror Bandpass Filter Sample Parameters / Fluorochromes Default Filters for Configuration BD_LSR-Fortessa_18-COLOR_20181211 Laser Detector Name Dichroic Mirror Bandpass Filter Sample Parameters / Fluorochromes 355C-379 None 379/28 BUV395 355B-474 450LP 474/25 CellTrace calcein Blue AM; L/D/F Blue; DC Violet; Hoechst 33342; Hoechst 33258; DAPI (best) 355A-730 685 LP 730/45 BUV737; Qdot 705 405E-450 BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor arrays. The BD LSRFortessa uses BD’s patented octagon- and trigon-shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options. Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) Filter 670/30 730/45 780/60 APC Alexa 700 APC-Cy7 Alexa 633 APC-Alexa 750 Alexa 635 APC-H7 (BD) Alexa 647 APC-eFluor 780 (eBioscience) Cy5 To-Pro-3 LIVE/DEAD Fixable Far Red (Invit.) eFluor 660 (eBio.) Vibrant DyeCycle Ruby (Invit.) Fortessa Configuration • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa. • Give the lasers 20-30 minutes of warm-up time. • Check tank levels for FACS buffer and waste o To fill FACS buffer unscrew probe and place in holster.

Collection filter, Mirror, Fluorochromes. 450/50, -, Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI,

(AF= Alexa Fluor). UV D. 379/28. BUV 395.